Most likely, yes.
Hi, I only experienced sex for like 3 times only. Does that affect the tightness of my vagina?
There are exercises one can do - Kegels.
The mayo clinic offers a step by step. These exercises offer a myriad of benefits. Some related to sex, and others just related to good health.
http://www.mayoclinic.org/healthy-lifestyle/womens-health/in-depth/kegel-exercises/art-20045283
It'll be a sausage up an alley now I'm afraid. No it won't affect it.
Of course it does!